Journal: Nature Communications

Article Title: Intracellular delivery of protein drugs with an autonomously lysing bacterial system reduces tumor growth and metastases

doi: 10.1038/s41467-021-26367-9

Figure Lengend Snippet: a ID Salmonella delivery of NIPP1-CD caused more death ( red , white arrows ) in Hepa 1-6 cells compared to controls ( P = 0.0021, n = 6). b In microfluidic tumor masses, delivery of NIPP1-CD caused more cell death ( red ) than bacterial controls. The percentage of dead cells increased with time as Salmonella invaded into cells and delivered protein ( P = 0.042, 0.017, 0.014, 0.017, 0.024, 0.030, and 0.039 at 6.5, 7, 7.5, 8, 8.5, 9, and 9.5 h, respectively, n = 4). c For multiple ( n = 4) tumor masses, as shown in b , NIPP1-CD significantly increased cell death ( P = 0.0177). d NIPP1-CD ID Salmonella was intravenously administered to BALB/c mice with subcutaneous 4T1 tumors. After 31 days, Salmonella ( black arrow , green ) and delivered NIPP1-CD ( white arrows , red ) were dispersed throughout the tissue. Data are shown as means ± SEM. Statistical comparisons in a – c are two-tailed, unpaired Student’s t -tests with asterisks indicating significance (* P < 0.05; ** P < 0.01). Images in a, b , and d are representative of 6, 4, and 3 independent biological samples, respectively. Scale bars in a and b are 100 µm, and in d the scale bar is 10 µm.

Article Snippet: After 31 days, tumors were excised and stained for Salmonella ( Abcam , catalog # ab69253; 1:100 dilution) and NIPP1-CD with antibodies to the c-terminal myc tag ( Chromotek , catalog # 9e1-100; 1:100 dilution), followed by a secondary antibody to the myc specific antibody ( Life Technologies , catalog # A11077; 1:100 dilution).

Techniques: Two Tailed Test

Journal: Ecology and Evolution

Article Title: Seasonal dynamics and co‐occurrence patterns of honey bee pathogens revealed by high‐throughput RT‐qPCR analysis

doi: 10.1002/ece3.5544

Figure Lengend Snippet: Primers used in this study

Article Snippet: Reference DNA of the type strains of Paenibacillus larvae (DSM‐7030) and Melissococcus plutonius (DSM‐29964) was acquired from the German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig, Germany).

Techniques: Virus, Bacteria, Control

Journal: Ecology and Evolution

Article Title: Seasonal dynamics and co‐occurrence patterns of honey bee pathogens revealed by high‐throughput RT‐qPCR analysis

doi: 10.1002/ece3.5544

Figure Lengend Snippet: Prevalence and abundance of pathogens and parasites in this study

Article Snippet: Reference DNA of the type strains of Paenibacillus larvae (DSM‐7030) and Melissococcus plutonius (DSM‐29964) was acquired from the German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig, Germany).

Techniques: Virus, Bacteria

Journal: Ecology and Evolution

Article Title: Seasonal dynamics and co‐occurrence patterns of honey bee pathogens revealed by high‐throughput RT‐qPCR analysis

doi: 10.1002/ece3.5544

Figure Lengend Snippet: Spearman's correlations of pathogen abundances in single forager bees calculated from log‐transformed concentration values. Zero values and measured concentrations below 1,000 target molecules/100 ng RNA were excluded from the analysis. Correlation values based on less than n = 50 data pairs are not shown. Significant (* p < .05) and highly significant correlations (** p < .001) are printed bold. The robustness of the correlations was tested by repeating the calculations with regional sub‐selections of the data. Correlations that were significant in all sub‐selections of the dataset are marked with a gray shades

Article Snippet: Reference DNA of the type strains of Paenibacillus larvae (DSM‐7030) and Melissococcus plutonius (DSM‐29964) was acquired from the German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig, Germany).

Techniques: Concentration Assay